Todd Przybycien Research and Publications

Publications: Refereed

Daly S, Przybycien TM, Tilton RD, "Adsorption of poly(ethylene glycol)-modified ribonuclease A to a poly(lactide-co-glycolide) surface," Biotechnology & Bioengineering 90, 856-868 (2005).

Daly S, Przybycien TM, Tilton RD (2005) "Adsorption of Polyethylene Glycol Modified Lysozyme to Silica," Langmuir 21, 1328-1337.

Sethuraman A, Vedantham G, Przybycien TM, Belfort G (2004) "Protein Unfolding at Interfaces: Slow Dynamics of -Helix to -Sheet Transition," Proteins: Structure, Function and Bioinformatics 56, 669-678.

Berglund KD, Truong MT, Tilton RD, Przybycien TM, Garoff SG, Walker LM (2004) "Rheology of Transient Networks Containing Hydrophobically Modified Cellulose, Anionic Surfactant and Colloidal Silica: Role of Selective Adsorption," Rheol. Acta 43, 50-61.

Sullivan MM, Green JJ, Przybycien TM (2003) "Development of a Novel Gene Delivery Scaffold Utilizing Colloidal Gold-Polyethylenimine Conjugates for DNA Condensation," Gene Therapy 10, 1882-1890.

Choi EJ, Foster MD, Daly S, Tilton RD, Przybycien TM, Majkrzak CF, Witte P, Menzel H (2003) "Effect of Flow on Human Serum Albumin Adsorption to Self-Assembled Monolayers of Varying Packing Density," Langmuir 19, 5464-5474.

Daly S, Przybycien TM, Tilton RD (2003) "Coverage Dependent Orientation of Lysozyme Adsorbed on Silica," Langmuir 19, 3848-3857.

Berglund KD, Przybycien TM, Tilton RD (2003) "Coadsorption of Sodium Dodecylsulfate with Hydrophobically Modified Nonionic Cellulose Polymers II: Role of Surface Selectivity in Adsorption Hysteresis," Langmuir 19, 2714-2721.

Berglund KD, Przybycien TM, Tilton RD (2003) "Coadsorption of Sodium Dodecylsulfate with Hydrophobically Modified Nonionic Cellulose Polymers I: Role of Polymer Hydrophobic Modification," Langmuir 19, 2705-2713.

Vedantham G, Carothers SL, Belfort G, Przybycien TM (2003) "Structural Response of Bovine Growth Hormone to Dead Ended Ultrafiltration," Separation Science and Technology 38, 251-270.

Berglund KD, Timko AE, Przybycien TM, Tilton RD (2003) "Use of Nonionic Ethylene Oxide Surfactants as Phase Transfer Catalysts for Poly(Acrylic Acid) Adsorption to Silica against an Electrostatic Repulsion," Progress in Colloid and Interface Science 122, 56-66.

Srinivasan U, Iyer GH, Przybycien TM, Samsonoff, WJ, Bell JA (2002) "Crystine: Fibrous Biomaterial from Protein Crystals Cross-linked in a Specific Geometry," Protein Engineering 15, 895-902. (featured on journal cover)

Doherty AS; Berglund KD; Buchholz BA; Kourkine IV; Przybycien TM; Tilton RD; Barron AE (2002) "Critical factors for high-performance physically adsorbed (dynamic) polymeric wall coatings for capillary electrophoresis of DNA," Electrophoresis 23, 2766-76.

Vedantham G, Sparks HG, Sane SU, Tzannis ST, Przybycien TM (2000) "A Holistic Approach for Protein Secondary Estimation from Infrared Spectra in H2O Solutions," Analytical Biochemistry 285, 33-49.

Bohme P, Vedantham G, Przybycien TM, Belfort G (1999) "Self-Assembled Monolayers on Polymer Surfaces: Kinetics, Functionalization and Photopatterning," Langmuir 15, 5323-5328.

Lam P, Kumar K, Wnek G, Przybycien TM (1999) "A Prototype Electrochemical Chromatographic Column for Use with Proteins," Analytical Chemistry 71, 4272-4277.

Lam P, Kumar K, Wnek G, Przybycien TM (1999) "Electroless Gold Plating of Stainless 316L Beads," Journal of the Electrochemical Society 146, 2517-2521.

Sane SU, Cramer, SM, Przybycien TM (1999) "Protein Structure Perturbations on Chromatographic Surfaces," Journal of Chromatography A 849, 149-159.

Sane SU, Cramer SM, Przybycien TM (1999) "A Holistic Approach to Protein Secondary Structure Characterization Using Amide I Band Raman Spectroscopy" Analytical Biochemistry 269, 255-272.

Winters MA, Frankel DZ, Debenedetti PG, Carey J, Devaney M, Przybycien TM (1999) "Protein Purification with Vapor-Phase Carbon Dioxide," Biotechnology and Bioengineering 62, 247-258.

Winters MA, Debenedetti PG, Carey J, Sparks HG, Sane SU, Przybycien, TM (1997) "Long-Term Storage of Supercritically-Processed Microparticulate Protein Powders," Pharmaceutical Research 14, 1370-1378.

Tzannis ST, Hrushesky WJM, Wood PA, Przybycien TM (1997) "Adsorption of Formulated Interleukin-2 on Drug Delivery Device Surfaces," Journal of Colloid and Interface Science 189, 216-228.

Patro SY, Przybycien TM (1996) "Self-Interaction Chromatography: A Tool for the Study of Protein-Protein Interactions in Bioprocessing Environments," Biotechnology & Bioengineering 52, 193-203.

Winters, MA, Knutson, BL, Debenedetti PG, Sparks HG, Przybycien TM, Stevenson CL, Prestrelski SS (1996) "Precipitation of Proteins in Supercritical Carbon Dioxide," Journal of Pharmaceutical Sciences 85, 586-594.

Tzannis ST, Hrushesky WJM, Wood PA, Przybycien TM (1996) "Irreversible Inactivation of Interleukin-2 in a Pump-based Delivery Environment," Proceedings of the National Academy of Sciences 85, 586-594.

Patro SY, Przybycien TM (1996) "Simulations of Reversible Protein Aggregate and Crystal Structure," Biophysical Journal 70, 2888-2902.

Iyer HV, Przybycien TM (1996) "A Model for Metal Affinity Protein Aggregation," Journal of Colloid and Interface Science 177, 391-400.

Lam P, Elliker PR, Wnek GE, Przybycien TM (1995) "Towards an Electrochemically Modulated Chromatographic Stationary Phase," Chromatography A 707, 29-33.

Iyer HV, Przybycien TM (1995) "Metal Affinity Protein Precipitation: Effect of Mixing and Modifiers on Fractionation," Biotechnology & Bioengineering, 48, 324-332.

Debenedetti PG, Yeo S-D, Patro SY, Przybycien TM (1994) "Secondary Structure Characterization of Microparticulate Insulin Powders," Journal of Pharmaceutical Sciences 83, 1651-1656.

Tzannis ST, Przybycien TM, Hrushesky WJM, Wood P (1994) "The Impact of Formulated Interleukin-2/Delivery Device Surface Interactions on Bioefficacy," in Biomaterials for Drug and Cell Delivery (Mikos, A.G., Murphy, R., Bernstein, H., and Peppas, N.A., eds.) MRS Symposium Proceedings 331, Materials Research Society, Pittsburgh, PA, pp 227-232.

Patro S, Przybycien TM (1994) "Simulations of Kinetically Irreversible Protein Aggregate Structure," Biophysical Journal 66, 1274-1289.

Iyer H, Przybycien TM (1994) "Protein Precipitation: Effects of Mixing on Protein Solubility," American Institute of Chemical Engineers Journal 40, 349-360.

Przybycien TM, Dunn JP, Georgiou G, Valax P (1994) "Secondary Structure Characterization of -Lactamase Inclusion Bodies," Protein Engineering 7, 131-136.

Storrs SB, Przybycien TM (1991) "Commercial Scale Refolding of Recombinant Bovine Somatotropin," in Protein Refolding (G. Georgiou and E. De Bernardez-Clark eds.) ACS Symposium Series No. 470, American Chemical Society, Washington, D.C., pp 197-205.

Przybycien TM, Bailey JE (1991) "Secondary Structure Perturbations in Salt-Induced Protein Precipitation," Biochimica et Biophysica Acta 1076, 103-111.

Przybycien TM, Bailey JE (1989) "Aggregation Kinetics in Salt-Induced Protein Precipitation," American Institute of Chemical Engineers Journal 35, 1779-1790.

Przybycien TM, Bailey JE (1989) "Structure-Function Relationships in the Inorganic Salt-Induced Precipitation of -Chymotrypsin," Biochimica et Biophysica Acta 995, 231-245.

Przybycien TM, Bailey JE (1989) "Solubility-Activity Relationships in the Inorganic Salt-Induced Precipitation of -Chymotrypsin," Enzyme and Microbial Technology 11, 264-276.

Publications: Other

S Daly, TM Przybycien, RD Tilton (2004) "Adsorption of PEGylated Proteins at the Solid/Liquid Interface," Biophysical Journal, 86, 624A, Part 2 Supplement

Review: Przybycien TM, Pujar NS, Steele LM (2004) "Alternative bioseparation operations: Life beyond packed bed chromatography," Current Opinion in Biotechnology 15, 469-478. (invited)

Review: Przybycien TM (1998) "Protein-Protein Interactions as a Means of Purification," Current Opinion in Biotechnology 9, 164-170. (invited)

Symposium Proceedings: Lam P, Przybycien TM, Kumar K, Wnek GE (1996) "Preparation of an Electrochemical Chromatographic Stationary Phase for Protein Separation," Proceed. 5th World Congress of Chemical Engineering, American Institute of Chemical Engineers, New York, NY

Book Review: Przybycien TM (1995) "Protein Purification Process Engineering" (edited by Roger Harrison), American Institute of Chemical Engineers Journal 41, 745-746. (invited)

Symposium Proceedings: Tzannis ST, Hrushesky WJM, Wood P, Przybycien TM (1994) "Protein-Surface Interactions Lead to Incomplete Delivery of Interleukin-2 with an Infusion Pump/Catheter System," Proceedings of the International Symposia on Controlled Release of Bioactive Materials 21, 361-362.

Book Review: Przybycien TM (1993) "Polymer Applications in Biotechnology" (edited by David S. Soane), Trends in Polymer Science 1, 93-94. (invited)

Publications: Submitted and In Press

Roy NK, Suter RM, Tilton RD, Przybycien TM, "Study of the conformational polymorphic transition in L-glutamic acid crystals," Crystal Growth and Design, submitted

Berglund KD, Tilton RD, Przybycien TM, "Anionic Polyelectrolyte Adsorption to Negatively Charged Surfaces via Association with Nonionic Surfactants: An ATR-FTIR Investigation," Journal of Colloid and Interface Science, submitted.

Patents and Disclosures

Hauan S, Neumann J, Bartkovsky M, Przybycien TM, Gabriel K, (2004) "MEMS Membrane Based Sensor," World Patent Application, International Publication No. WO 2004/044569, 27 May.

Hauan S, Przybycien TM, Gabriel K, Neumann J, Bartkovsky M (2003) "MEMS Membrane Based Sensor," US Patent Application No. 10/702,709, 6 November.

Hauan S, Przybycien TM, Gabriel K, Neumann J, Bartkovsky M (2002) "A MEMS-based Biosensor," Provisional Patent Application 60/424,125, 6 November.

Lam P, Wnek GE, Przybycien TM (1999) "Electrical Impedance Sensor for Protein-Surface Interactions and Application to Protein Aggregation in Drug Formulation," Technology Transfer Office, Virginia Commonwealth University Disclosure

Przybycien TM, Lam P, Wnek GE, Elliker P (1998) "Electrochemical Separation Utilizing Metalloporphyrins and Metallophthalocyanines," U.S. Patent No. 5,711,867, 27 January.