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Research Interests
Our group addresses the practical problems and underlying fundamental phenomena associated with the production, formulation, and delivery of pharmaceutical proteins generated by the biotechnology industry. We are also pursuing the development of protein-based nanomaterials and the formulation of nucleic acids for gene therapy as extensions of our expertise in applied biophysics.
Protein/Nucleic Acid Aggregation Topics
- Protein-protein interactions in refolding, separation, finishing, and formulation environments
- Mixing phenomena and the rational design and scaleup of protein precipitation operations
- Characterization of proteins in aggregate states
- Screening and rational selection of excipients for protein and nucleic acid formulations
- Development of protein aggregates and crystalline arrays for use as nanomaterials.
Protein Adsorption Topics
- Interactions between formulated therapeutic proteins and drug delivery device surfaces and attendant bioefficacy issues
- Structural characterization of proteins adsorbed on chromatographic and membrane separations media.
Biophysical Methods Topics
- Development of Raman and infrared spectroscopy for protein secondary structure estimation
- Characterization of protein-protein interactions via low frequency Raman and Brillouin spectroscopy
- Development of macroscopic and MEMS-based microgravimmetric assay techniques for protein adsorption and protein-protein interaction measurements
Representative Publications
Tzannis, S.T., Hrushesky, W.J.M., Wood, P.A. and Przybycien, T.M. "Irreversible Inactivation of Interleukin-2 in a Pump-based Delivery Environment," PNAS vol. 85, p. 586, 1996.
Patro, S.Y. and Przybycien, T.M. "Self-Interaction Chromatography: A Tool for the Study of Protein-Protein Interactions in Bioprocessing Environments," Biotechnol. Bioeng. vol. 52, p. 193, 1996.
Sane, S.U., Cramer, S.M. and Przybycien, T.M. "A Holistic Approach to Protein Secondary Structure Characterization Using Amide I Band Raman Spectroscopy" Analytical Biochemistry vol. 269, p. 255, 1999.
Sane, S.U., Cramer, S.M. and Przybycien, T.M. "Protein Structure Perturbations on Chromatographic Surfaces," Journal of Chromatography A vol. 849, p. 149, 1999.
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